Journal: Materials & Design

Article Title: Multifunctional nanofiber-based dressings in coordination with adipose-derived stem cells for accelerated burn wound healing

doi: 10.1016/j.matdes.2025.113929

Figure Lengend Snippet: Fig. 4. (A) Detection of ADSCs proliferation by ADM coating at different times. Quantification of (B) EGF and (C) FGF2 in ADSCs paracrine products. *** P < 0.001 representing a significant difference as compared with ADM (3 min) and TLWDA.

Article Snippet: Human EGF enzyme-linked immunosorbent assay (ELISA) kit and human FGF2 ELISA kit were purchased from BOSTER (Wuhan, China).

Techniques:

Journal: Oncogene

Article Title: Transduction of the SkBr3 breast carcinoma cell line with the HOXB7 gene induces bFGF expression, increases cell proliferation and reduces growth factor dependence.

doi: 10.1038/sj.onc.1201875

Figure Lengend Snippet: Figure 1 Expression of HOXB7 and bFGF genes in A375 melanoma, SkBr3 mammary carcinoma and HOXB7-transduced SkBr3. (a) RNase protection analysis. Riboprobes (R) and protected fragments (P) are shown on the right, and molecular size (base pairs) markers (MWM) (pGEM4Z HpaII) on the left. b-actin expression is shown as internal control. (b) Northern blot analysis. b2 microglobulin expression is shown as internal control. (c) Western blot analysis of bFGF produced by A375 melanoma, SkBr3 mammary carcinoma and HOXB7-transduced SkBr3 cells

Article Snippet: Intracellular and secreted bFGF were quanti®ed by a human bFGF ELISA from R&D systems (Minneapolis, MN).

Techniques: Expressing, Control, Northern Blot, Western Blot, Produced

Journal: Oncogene

Article Title: Transduction of the SkBr3 breast carcinoma cell line with the HOXB7 gene induces bFGF expression, increases cell proliferation and reduces growth factor dependence.

doi: 10.1038/sj.onc.1201875

Figure Lengend Snippet: Figure 3 (a) Role of bFGF for SkBr3/HOXB7 cell growth in low serum. Growth kinetics of parental and HOXB7-transduced SkBr3 cells maintained in 10% or 1% FCS were compared. Cell growth was monitored over the indicated time intervals by methylene blue inclusion, as indicated in Materials and methods. (b) Eect of exogenous rbFGF on SkBr3 cell growth. Growth curves of cells maintained in 1% FCS plus dierent concentra- tions of rbFGF evaluated by methylene blue inclusion

Article Snippet: Intracellular and secreted bFGF were quanti®ed by a human bFGF ELISA from R&D systems (Minneapolis, MN).

Techniques:

Journal: Oncogene

Article Title: Transduction of the SkBr3 breast carcinoma cell line with the HOXB7 gene induces bFGF expression, increases cell proliferation and reduces growth factor dependence.

doi: 10.1038/sj.onc.1201875

Figure Lengend Snippet: Figure 4 Inhibition of SkBr3/HOXB7 cell proliferation upon treatment with 30 mM of bFGF antisense (a) or of sense (s) oligomers. Evidence of bFGF intracrine loop operating in cells kept in low serum. Mean+s.d. of three separate experiments is shown

Article Snippet: Intracellular and secreted bFGF were quanti®ed by a human bFGF ELISA from R&D systems (Minneapolis, MN).

Techniques: Inhibition